Installing Crystallography Software
CCP4 (includes Coot): https://www.ccp4.ac.uk/download/#os=macos
Phenix: https://phenix-online.org/download
Tips For DNA Oligos
Resuspending DNA Oligos (IDT DNA Tips)https://www.idtdna.com/pages/education/decoded/article/tips-for-resuspending-and-diluting-your-oligonucleotides
DNA Oligo Resuspension Calculator: https://www.idtdna.com/Calc/resuspension/
IDT DNA OligoAnalyzer (SS-structure, Tm, extinction coefficients etc.): https://www.idtdna.com/calc/analyzer
DNA and RNA Molecular Weights and Conversions: https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rna-tools-and-calculators/dna-and-rna-molecular-weights-and-conversions.html
Crystallization Resources
Beamline Guides (MiTeGen):
All: https://www.mitegen.com/learn/beamline-guides/
ALS 8.2.2: https://www.mitegen.com/learn/beamline-guides/als-8-2-2/
NSLS II/AMX: https://www.mitegen.com/learn/beamline-guides/nsls-ii-amx-17-id-1/
Crystal Freezing
Servers:
ProtParam- https://web.expasy.org/protparam/
A tool which allows the computation of various physical and chemical parameters for a given protein. The computed parameters include the molecular weight, theoretical pI, amino acid composition, atomic composition, and extinction coefficient.
JANE- Journal Author Name Estimator – https://jane.biosemantics.org/index.php
Enter the title and/or abstract of your potential paper in the box, and Jane will compare it to find the best matching journals, authors or articles to help choose a place for submission.
PDBePISA (Proteins, Interfaces, Structures and Assemblies)– https://www.ebi.ac.uk/msd-srv/prot_int/pistart.html
Results include:
- Structural and chemical properties of macromolecular surfaces and interfaces
- probable quaternary structures (assemblies), their structural and chemical properties and probable dissociation pattern
PDBeFold- https://www.ebi.ac.uk/msd-srv/ssm/
A comparison with other protein matching services. PDBeFold is used as a structure search engine in PDBePISA.
Dali – http://ekhidna2.biocenter.helsinki.fi/dali/
The Dali server is a network service for comparing protein structures in 3D.