{"id":80,"date":"2018-02-03T13:40:29","date_gmt":"2018-02-03T18:40:29","guid":{"rendered":"http:\/\/pages.vassar.edu\/institutionalbiosafety\/?page_id=80"},"modified":"2018-02-03T14:28:32","modified_gmt":"2018-02-03T19:28:32","slug":"risk-groups","status":"publish","type":"page","link":"https:\/\/pages.vassar.edu\/institutionalbiosafety\/risk-groups\/","title":{"rendered":"Risk Groups"},"content":{"rendered":"

Risk Group One (RG-1)<\/strong>
\n<\/span><\/span>
\n<\/span>Risk Group One (RG-1) agents are usually not placed on a list but include all microorganisms that do not pose a health risk to healthy adult humans. It must not be assumed that an organism not listed as a RG 2, 3, or 4 agent is an RG-1 agent; emerging or unknown organisms should be treated as biohazardous until research proves otherwise. Examples of agents in RG-1 are: Bacillus subtilus, infectious canine hepatitis viruses; influenza reference strains A\/PR\/8\/34, A\/WS\/33, Escherichia coli K12, Saccharomyces cerevisiae, and other agents listen in Appendix C-II of the NIH Guidelines for Research Involving Recombinant DNA Molecules; and other vectors such as Baculovirus.<\/span>
\n<\/span><\/p>\n

E. coli strains used for cloning and gene expression are typically RG1. Use of strains derived from strain K12 is exempt from IBC approval but must still be registered with the IBC. Other strains of E. coli not derived from K12 are likely RG1 but are not exempt from approval. Is my strain derived from K12?\u00a0<\/a><\/p>\n


\n<\/span>Risk Group Two (RG-2)
\n<\/strong>
\n<\/span>RG-2 agents are of moderate potential hazard to healthy adult humans and the environment. Such agents may produce disease of varying degrees of severity from accidental inoculation, injection, or other means of cutaneous penetration but can usually be adequately and safely contained by ordinary laboratory techniques. Some agents may cause disease by contact or respiratory routes, but they are self-limiting and do not cause a serious illness, such as the cause of the common cold, the rhinoviruses. The following organisms have been identified as RG-2 agents:<\/span>
\n<\/span>
\n<\/span>Bacteria<\/span>
\n<\/span>
\n<\/span>Acinetobacter baumannii<\/span>
\n<\/span>Actinobacillus spp \u00a0<\/span>
\n<\/span>Actinomyces pyogenes<\/span>
\n<\/span>Aeromonas hydrophila<\/span>
\n<\/span>Amycolata autotrophica<\/span>
\n<\/span>Archanobacterium haemolyticum<\/span>
\n<\/span>Bacteroides spp.<\/span>
\n<\/span>Borrelia recurrentis <\/span>
\n<\/span>Burkholderia (except those in RG-3)<\/span>
\n<\/span>Campylobacter coli<\/span>
\n<\/span>Clostridium chauvoei<\/span>
\n<\/span>Corynebacterium diphtheriaDermatophilus congolensis<\/span>
\n<\/span>Edwardsiella tarda<\/span>
\n<\/span>Erysipelothrix rhusiopathiae<\/span>
\n<\/span>Escherichia coli<\/span>
\n<\/span>Haemophilus ducreyi<\/span>
\n<\/span>Helicobacter pylori<\/span>
\n<\/span>Klebsiella spp.<\/span>
\n<\/span>Leptospira interrogans<\/span>
\n<\/span>Listeria spp.<\/span>
\n<\/span>Moraxella spp.<\/span>
\n<\/span>Mycobacterium spp.<\/span>
\n<\/span>Nocardia asteroidsPasteurella spp.<\/span>
\n<\/span>Plesiomonas shigelloides<\/span>
\n<\/span>Proteus spp.<\/span>
\n<\/span>Salmonella spp.<\/span>
\n<\/span>Sphaerophorus necrophorus<\/span>
\n<\/span>Staphylococcus aureus<\/span>
\n<\/span>Streptobacillus moniliformis<\/span>
\n<\/span>Streptococcus spp<\/span>
\n<\/span>Treponema pallidum<\/span>
\n<\/span>Vibrio cholera<\/span>
\n<\/span>Yersinia enterocolitica<\/span>
\n<\/span>
\n<\/span>Fungal Agents<\/span>
\n<\/span>
\n<\/span>Blastomyces dermatitidis<\/span>
\n<\/span>Cladosproium bantianum<\/span>
\n<\/span>Cryptococcus neoformans<\/span>
\n<\/span>Epidermophyton spp. Exophiala dermatitidis<\/span>
\n<\/span>Fonsecaea pedrosoi<\/span>
\n<\/span>Microsporum spp.<\/span>
\n<\/span>Ochroconis gallopavumParacoccidiodes brasilensis<\/span>
\n<\/span>Penicillium marneffei<\/span>
\n<\/span>Sporothrix schenckii<\/span>
\n<\/span>Trichophyton spp.<\/span><\/p>\n


\n<\/span>LOW RISK AGENTS<\/span><\/p>\n

In addition to the examples listed above, the following low-risk oncogenic viruses have been identified as RG-1 agents:<\/p>\n

Adenvirus 7-Simian virus 40 (Ad7-SV40)
\nAvian Leucosis virus
\nBovine leukemia virus
\nBovine papilloma virus
\nChick-embryo-lethan orphan (CELO) virus
\nDog sarcoma virus
\nGuinea pig herpes virus
\nLucke (frog) virus
\nHamster leukemia virus
\nMarek\u2019s disease virus
\nMason-Pfizer monkey virus
\nMouse mammary tumor virus
\nMurine leukemia virus
\nMurine sarcoma virus Polyoma virus
\nRat leukemia virus
\nRous sarcoma virus
\nShope vibroma virus
\nShope papilloma virus
\nSimian virus 40 (SV-40)
\n
\n<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Risk Group One (RG-1) Risk Group One (RG-1) agents are usually not placed on a list but include all microorganisms that do not pose a health risk to healthy adult humans. It must not be assumed that an organism not listed as a RG 2, 3, or 4 agent is an RG-1 agent; emerging or … Continue reading “Risk Groups”<\/span><\/a><\/p>\n","protected":false},"author":315,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-80","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pages.vassar.edu\/institutionalbiosafety\/wp-json\/wp\/v2\/pages\/80","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pages.vassar.edu\/institutionalbiosafety\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pages.vassar.edu\/institutionalbiosafety\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pages.vassar.edu\/institutionalbiosafety\/wp-json\/wp\/v2\/users\/315"}],"replies":[{"embeddable":true,"href":"https:\/\/pages.vassar.edu\/institutionalbiosafety\/wp-json\/wp\/v2\/comments?post=80"}],"version-history":[{"count":3,"href":"https:\/\/pages.vassar.edu\/institutionalbiosafety\/wp-json\/wp\/v2\/pages\/80\/revisions"}],"predecessor-version":[{"id":85,"href":"https:\/\/pages.vassar.edu\/institutionalbiosafety\/wp-json\/wp\/v2\/pages\/80\/revisions\/85"}],"wp:attachment":[{"href":"https:\/\/pages.vassar.edu\/institutionalbiosafety\/wp-json\/wp\/v2\/media?parent=80"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}