{"id":476,"date":"2017-04-13T20:36:47","date_gmt":"2017-04-14T00:36:47","guid":{"rendered":"http:\/\/pages.vassar.edu\/bergstromlab\/?page_id=476"},"modified":"2022-11-16T15:35:34","modified_gmt":"2022-11-16T20:35:34","slug":"imaging","status":"publish","type":"page","link":"https:\/\/pages.vassar.edu\/bergstromlab\/imaging\/","title":{"rendered":"Imaging & Lab Tools"},"content":{"rendered":"

Engram “tagging”<\/strong><\/p>\n

Medial prefrontal cortex tissue immunolabeled for GFP (green), Arc\/arg3 (red) and DAPI (blue) using a 20X objective. Co-labeled neuron indicated by arrowhead – the \u201cengram cell\u201d\u00a0<\/strong><\/p>\n

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Gi-DREADD (hM4Di) transfection (mCherry) and c-fos staining in the mPFC following fear extinction retrieval.<\/strong><\/p>\n

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In vivo<\/em> calcium imaging using miniature\u00a0microscopes<\/b><\/p>\n

Infralimbic cortex under conditions that promote behavior extinction.<\/strong><\/p>\n

0.5 mm diameter, 6.1 mm length GRIN. AAV.CamKII.GCamP6s (AAV9).<\/strong><\/p>\n

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The miniscope<\/strong><\/p>\n

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DREADDs (pAAV-CaMKIIa-EGFP)<\/span><\/strong><\/p>\n

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Golgi-stain<\/strong><\/p>\n

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Golgi-stained BLA principal neuron (20 X)<\/p>\n

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Golgi-stained BLA principal neurons (20 X)<\/p><\/div>\n

Arc\/arg 3.1 immunohistochemistry<\/strong><\/p>\n

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c-fos immunofluorescence<\/strong><\/p>\n

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\u00a03D recontruction of a principal neurons in the basolateral amygdala\"\"<\/a><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Engram “tagging” Medial prefrontal cortex tissue immunolabeled for GFP (green), Arc\/arg3 (red) and DAPI (blue) using a 20X objective. Co-labeled neuron indicated by arrowhead – the \u201cengram cell\u201d\u00a0 Gi-DREADD (hM4Di) transfection (mCherry) and c-fos staining in the mPFC following fear … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":5713,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-476","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pages.vassar.edu\/bergstromlab\/wp-json\/wp\/v2\/pages\/476","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pages.vassar.edu\/bergstromlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pages.vassar.edu\/bergstromlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pages.vassar.edu\/bergstromlab\/wp-json\/wp\/v2\/users\/5713"}],"replies":[{"embeddable":true,"href":"https:\/\/pages.vassar.edu\/bergstromlab\/wp-json\/wp\/v2\/comments?post=476"}],"version-history":[{"count":20,"href":"https:\/\/pages.vassar.edu\/bergstromlab\/wp-json\/wp\/v2\/pages\/476\/revisions"}],"predecessor-version":[{"id":1016,"href":"https:\/\/pages.vassar.edu\/bergstromlab\/wp-json\/wp\/v2\/pages\/476\/revisions\/1016"}],"wp:attachment":[{"href":"https:\/\/pages.vassar.edu\/bergstromlab\/wp-json\/wp\/v2\/media?parent=476"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}