Imaging & Lab Tools

In vivo calcium imaging using miniature microscopes

Infralimbic cortex under conditions that promote behavior extinction.

0.5 mm diameter, 6.1 mm length GRIN. AAV.CamKII.GCamP6s (AAV9).

The miniscope

 

DREADDs (pAAV-CaMKIIa-EGFP)

Golgi-stain

 

Golgi-stained BLA principal neuron (20 X)

Golgi-stained BLA principal neurons (20 X)

Arc/arg 3.1 immunohistochemistry

 

c-fos immunofluorescence

 3D recontruction of a principal neurons in the basolateral amygdala